It was again extracted with ether three times. The resulting organic layer was washed with brine, and then dried with MgSO4 to remove excess water. Gravity filtration was set up to remove the MgSO4. The product was concentrated and purified by flash chromatography (gradient, first pure hexanes, then 5% to 10 % to 15 % v/v EtOAc/Hexanes) to produce product 26 in a white solid form.

Gravity filtration – This involves putting a piece of filter paper over a stemless funnel.  Hot solution is poured over the product on the filter paper.  The product will redissolve into the solvent and go through the paper while the impurities will not.


Flash chromatography–This involves using nitrogen gas to add pressure to a column in column chromatography to make the solvent run faster.  Column chromatography involves running the product through a vertical column using an eluent normally composed of hexane and ethyl acetate.  Lower polarity products will come out of the bottom of the column before the more polar products allowing separation of products with different polarities.