EXAMPLE CD7-5
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![]() Determine the figure that corresponds to each of the three types of inhibition: competitive, uncompetitive, and noncompetitive. |
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SOLUTION | ||||||
For competitive inhibition: | ||||||
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Multiplying by and rearranging![]() |
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We see that a plot of rp
vs. rp /S will be a straight
line with a slope![]() |
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A change in the inhibitor concentration will change the slope of the Eadie plot but not the intercept. The greater the inhibitor concentration, the greater (i.e., steeper) the slope. | ||||||
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For uncompetitive inhibition: | ||||||
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After rearranging (CD7-16) so that | ||||||
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we see that increasing the inhibitor concentration will decrease the slope and the intercept of an Eadie plot. | ||||||
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For noncompetitive inhibition: | ||||||
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Again,multiplying by (S + K m) and rearranging, we obtain | ||||||
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In the case of noncompetitive inhibition, increasing the inhibitor concentration decreases the intercept but does not affect the slope of an Eadie plot. | ||||||
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