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Chapter 9: Reaction Mechanisms, Pathways, Bioreactions and Bioreactors
Additional Homework Problems
CDP9-HC
The enzymatic hydrolization of fish oil extracted from crude eel oil has been
carried out using lipase L (Proc. 2nd Joint China/USA Chemical Engineering
Conference. Vol.III, p. 1082, 1997). One of the desired products is
docosahexaenic acid, which is used as a medicine in China. For 40 mg of enzyme
the Michaelis constant is 6.2 X 10-2 (ml/ml) and Vmax is
5.6 . Calculate the
time necessary to reduce the concentration of fish oil from 1.4% to 0.2 vol%.
Note: There may be inconsistency in the article. The half life for an initial
volume of 25% fish oil is stated to be 4.5 days. However, this yields a
different initial fish oil concentration one finds from looking in the
literature. Search the web for fish oil. Suggest a way to resolve this
controversy.
[3rd Ed. P7-10]