LightCycler 480

    The LightCycler 480 is a state-of-the-art real-time PCR system capable of running both 96-well and 384-well formats in around 40 minutes.  There are a number of software modules that we have available including the Relative Quantification, Absolute Quantification, and Gene Scanning packages.  A data station is also available for non-run related data analysis.

Microbiome Resources

Resources:

1. LightCycler 480

    Real-Time PCR Machine        


2. Nanodrop Spectrophotometer


3. Anaerobic Chamber


4. Computer Workstations



Other Resources


  K9

  our analysis toolkit


How to Analyze

T-RFLP Data Using Peak Scanner



University Resources

  1. DNA Sequencing Core

  2. Biomedical Mass Spectrometry Core

  3. Ribosomal Database Project

 



        

Nanodrop Spectrophotometer

    The NanoDrop® ND-1000 UV-Vis Spectrophotometer enables highly accurate analyses of 1 ul samples with remarkable reproducibility. The patented sample retention system eliminates the need for cuvettes and capillaries which decreases the measurement cycle time. In addition, the high absorbance capability eliminates the need for most dilutions.

    





Anaerobic Chamber

      Description forthcoming.




Computer Workstations


                              

                                                                  


    One key aspect of microbiome research is that it is very computationally intensive.  To assist users to meet this challenge we have set up workstations computers with software to analyze T-RFLP, Clone Library and Microbiological Community data.  Available software includes Peak Scanner (a replacement for GeneScan when analyzing T-RFLP files), R, DOTUR, SONS, Treeclimber, S-Libshuff and K9 an in-house designed data analysis toolbox.  Additional methods of analysis are frequently updated as the needs of our users expand.