D2O Exchange With WT and Glutamine Mutants
The phosphorescence decay of WT and mutant AP mixed into
D2O buffer is biphasic. During the course
of an experiment the same two lifetimes are always observed, however, the
relative contribution each lifetime makes to the total phosphorescence
decay changes. Such behavior is indicative of a two-state reaction.
Figure 2a: Change in relative percentages of long and short
lifetime components in the average RTP lifetime of D2O-exchanged
WT AP at 60 °C The phosphorescence decay of WT under these conditions
is best fit by two exponentials: a 120 ms component and a 240 ms component.
Figure 2b: A MEM distributional analysis
of data taken 10 min. after mixing.
Phosphorescence Activation
Energies