Analysis of protein secondary structure is commonly
accomplished by measuring the chiral absorbance of far Ultraviolet
(far-UV) due to the sensitivity of the peptide chromophore
backbone.
Subtle changes in protein secondary structure induced by
amino acid substitutions of Plasminogen Activator Inhibitor are
followed by Circular Dichroism
Analysis of protein secondary structure has been actively persued by a
number of groups (Fasman, etc links).