772 Hydrogel Drug Delivery Vehicle Cytotoxicity for Pulp Capping in vitro    

Friday, March 23, 2012: 2 p.m. - 3:15 p.m.
Presentation Type: Poster Session
T. KOMABAYASHI1, A. WADAJKAR2, C. AHN3, Q. ZHU4, L.A. OPPERMAN5, L.L. BELLINGER5, J. YANG2, and K.T. NGUYEN2, 1Dept. of Endodontics, Texas A&M Health Science Center Baylor College of Dentistry, Dallas, TX, 2Dept. of Bioengineering, University of Texas Arlington, Arlington, TX, 3Dept. of Clinical Science, University of Texas Southwestern Medical Center at Dallas, Dallas, TX, 4Dept. of Endodontology, University of Connecticut School of Dental Medicine, Farmington, CT, 5Dept. of Biomedical Sciences, Texas A&M Health Science Center Baylor College of Dentistry, Dallas, TX
Objectives: Direct pulp capping is a treatment for preserving the vital pulp. However, a satisfactory material for caries-exposed inflamed pulp in permanent teeth is not currently available. Thus, a biologically-based material that promotes the continued formation of a new dentin-pulp complex is needed. A hydrogel drug delivery vehicle is a promising material, however its cytotoxicity has barely been explored. The aim of this study is to evaluate the cytotoxicity of a hydrogel drug delivery vehicle and its components in a cell culture system in vitro.          

Methods: The hydrogel solution was prepared by mixing poly(ethylene glycol)maleate-citrate (45% w/v)[PEGMC], acrylic acid (5% w/v)[AA], 2,2’-Azobis(2-methylpropionamidine) dihydrochloride photoinitiator (0.1% w/v)[AAPH], and deionized water. Various concentrations of the hydrogel and its components were prepared in the cell culture medium. L929 cells were seeded into 96-well plates at 3x103 cells/well and cultured with each concentration of the tested materials. Cells cultured only with the culture medium served as a control. After incubation the cytotoxicity was evaluated by MTS assay. The data was analyzed by ANOVA, Student’s t-tests for pair-wise comparisons between groups.

Results: For the hydrogel vehicle, cell viability dropped to 46.3% at 25% concentration (p=0.002); there was no cell survival at 100% concentration (p<0.0001). In PEGMC, cell viability dropped to 2.56% at 0.352 % concentration (p=0.006); there was no cell survival at 1.41% to 45% concentration (p=0.005). In AA, no cell survival observed at all concentrations. In AAPH, cell viability remained 100% up to 1% concentration. However, cell viability dropped to 15.6% at 2% concentration (p=0.002); there was no cell survival at 4% concentration (p<0.0001).

Conclusions: When redesigning hydrogel vehicle for dental pulp, its cytotoxicity and the individual component amounts within their biocompatibility range should be considered. Supported by NIH KL2RR024983(TK)/UL1RR024982.

This abstract is based on research that was funded entirely or partially by an outside source: NIH KL2RR024983(TK)/UL1RR024982

Keywords: Biocompatibility, Dentin, Endodontics, Hydrogel and Pulp
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