1139 Survival of PDL Cells in Different Storage Medias and times

Friday, March 23, 2012: 3:30 p.m. - 4:45 p.m.
Presentation Type: Poster Session
S. SHAHRABI FARAHANI, Oral pathology, Harvard School of Dental Medicine, Boston, MA, A. NAVABAZAM, Oral and Maxillofacial surgery, Dental School of Shahid Sadoughi University and Health Services, Yazd, Iran, A. MOMEN, Periodontology, Dental School of Shahid Sadoughi University and Health Services, Yazd, Iran, H. GHASEMPOUR, Dental School of Shahid Sadoughi University and Health Services, Yazd, Iran, and M.M. IMANI EMADI, Soheil Dentistry and Dental Implant Center, Tehran, Iran
"Objectives: " Vitality of periodontal ligament (PDL) cells is very critical for replantation of complete avulsion teeth due to traumatic injuries. The purpose of this study was to compare the vitality of PDL cells of sheep teeth cultured in different storage medias including α-Minimum Essential Medium (αMEM), Dulbecco’s Modified Eagle’s Medium (DMEM), Hank’s Balanced Salt Solution (HBSS), and mint extract for different incubation times of 2, 6, 24, 48, 72, or 96 hours.

"Methods: " In this lab trial study, PDL cells were obtained from 124 healthy anterior and posterior sheep teeth and cultured in αMEM, DMEM, HBSS, and mint extract for periods of 2, 6, 24, 48, 72, or 96 hours (24 groups). For each solution, positive control group was considered those evaluated PDL cells without incubation in any storage condition. For each group, there was a negative control considered cells growing in dry plate with no medium. After exposure of PDL cells to scheduled solution for scheduled incubation time, centrifuge was performed for 10 minutes. Then collagenase (3mgr/ml) and Dispase (4mgr/ml) were added to cell precipitates which were incubated at 37° C for 60 minutes. After washing cellular suspension in PBS, vitality of the cells was assessed by Trypan blue exclusion. The data was analyzed statistically using 2-way ANOVA test.

"Results: " Statistically significant differences in efficacy of different medias were obtained at least between two conditions (P=0.0001). PDL cells cultured in αMEM and mint extract showed 90% and 52.22% vitality representing the best and the worst storage media, respectively.

"Conclusions: " αMEM can be a suitable transport medium up to 96 hours to preserve the vitality of the PDL cells of avulsed teeth. There is a reverse correlation between the viability of PDL cells and incubation time.

This abstract is based on research that was funded entirely or partially by an outside source: Research and clinical center for infertility, Yazd, IRAN

Keywords: Animal, Cell culture, Periodontium-gingiva, Teeth and storage media