1246 Microleakage study of fluoride-releasing sealants

Saturday, March 24, 2012: 9:45 a.m. - 11 a.m.
Presentation Type: Poster Session
E. LEE, Comprehensive Dentistry and Biomaterials--Dental Biomaterials, Louisiana State University, New Orleans, LA, J.A. TOWNSEND, 1100 Florida Ave, Louisiana State University, New Orleans, LA, Y. FAN, Department of Comprehensive Dentistry and Biomaterials, Louisiana State University, New Orleans, LA, J.L. HAGAN, Biostatistics, Louisiana State University, New Orleans, LA, and X. XU, Comprehensive Dentistry & Biomaterials, Louisiana State University, New Orleans, LA
Objective: This study aims to investigate the adhesion and microleakage of experimental and commercial antibacterial and fluoride-releasing sealants. Two experimental sealants (EXP-1, EXP-2) developed by LSUSD contain an antibacterial fluoride-releasing monomer. EXP-2 contains additional bioactive glass filler.  Fluoride releasing commercial sealants Clinpro (3M ESPE, CL) and Fluroshield (Caulk/Dentsply, FS), and SeLECT Defense sealant (E34 Technologies), a non-fluoride releasing sealant containing an antimicrobial selenium compound, were also included for comparison.

Method: 5 groups of extracted teeth (6 maxillary molars and 4 mandibular molars per group) were randomly sorted and cleaned. A 0.5-1mm fissure was made in each sample, followed by etching (30s), cleaning, and drying. Sealants were applied according to manufacturer's instructions. The samples were light cured 40s and checked with an explorer. The unsealed surface of the sample was coated with a nail-polish and thermocycled 5°C-55°C for 2,000 cycles. The specimens were stained in 2% basic fuchsin for 24hrs, rinsed, air dried,and embedded in epoxy-resin. Each specimen was then sectioned mesiodistally into 6 slabs (1.2 mm-thick, 5-7 surfaces), observed and photographed under a microscope at 40x magnification. The microleakage degrees were scored on a scale of 0 to 4 (0=no penetration, 1=1/4 penetration, 2=1/2 penetration, 3=3/4 penetration, 4=penetration into the bottom of the fissure). The data was analyzed using the Kruskal-Wallis test with Tukey’s Honestly Signficant Differences test performed on the ranks for post-hoc analysis.

Result: The Kruskal-Wallis test revealed a significant difference (Χ2=21.0, df=4, p<0.001) between scores for different sealants.  The mean scores and standard deviations were: EXP-2 (0.69±0.58), EXP-1 (1.48±0.64), CL (2.19±1.06), E34 (2.49±0.96), and FS (2.62±1.03). EXP-2 and EXP-1 had significantly lower scores and smaller standard deviation than other groups (p<0.01).

Conclusion: Both the EXP-1 and EXP-2 sealants showed little to no leakage compared to commercial sealants. Therefore, they are expected to have excellent retention. Supported by NIH/NIDCR grant 5R01DE019203-03.

This abstract is based on research that was funded entirely or partially by an outside source: NIH/NIDCR grant 5R01DE019203-03

Keywords: Adhesion, Dental materials, Sealants and Sealants