124 Role of PLUNC in diabetes and oral disease

Thursday, March 22, 2012: 8 a.m. - 9:30 a.m.
Presentation Type: Oral Session
S. BENCHARIT1, S. SCHWARTZ2, J. CARLSON2, H. KOHLTFARBER3, C.R. MACK4, R. TARRAN5, and M.R. REDINBO6, 1Prosthodontics, University of North Carolina, Chapel Hill, NC, 2David H. Murdock Research Institute, Kannapolis, NC, 3Diagnostic Sciences, University of North Carolina, Chapel Hill, NC, 4Department of Dental Research, University of North Carolina, Chapel Hill, NC, 5Cystic Fibrosis Center, University of North Carolina, Chapel Hill, NC, 6Chemistry, University of North Carolina, Chapel Hill, NC
Objectives: Palate, lung, and nasal epithelium clone protein (PLUNC) is a secreted protein in saliva, upper respiratory tract, and lung. PLUNC is known to associated with several oral conditions including diabetes and periodontitis.

Methods: Using computational and proteomic methods, we define the structural domain of PLUNC and propose how PLUNC may function in saliva. Using mass spectrometry proteomics, we compared (1) the salivary proteomes from patients with diabetes and non-diabetic patients; and (1) the salivary proteomes from HIV patients with and without oral candidiasis. Multidimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS) was used. Cluster analysis and principle component analysis were used to define the protein biomarkers.

Results: PLUNC appears to have a globular structure similar to bactericidal/permeability-increasing proteins (BPI) and lipopolysaccharide-binding proteins (LBP). The N-terminal portion of PLUNC is unstructured. Mass spectrometry proteomics suggest that PLUNC is among the most prominent biomarkers found associated with diabetes and oral candidiasis associated with HIV-infection.

Conclusions: PLUNC plays an important role in oral immunity. PLUNC may prevent oral infection in patients with  diabetes and prevent candidal opportunistic infection in HIV-infected patients.

This abstract is based on research that was funded entirely or partially by an outside source: NIH grants: HL092338 and UL1RR025747; and NCTraCS grants: 2KR80905 and 10KR81002

Keywords: Diabetes, Diagnosis and Saliva
<< Previous Abstract | Next Abstract