397 Differences of Subgingival Microbiota between Young and Elder Rhesus Macaques

Thursday, March 22, 2012: 2 p.m. - 3:15 p.m.
Presentation Type: Poster Session
C.M. MURPHY1, A.S. KOKARAS1, B.J. PASTER1, and M. GEORGE2, 1Department of Molecular Genetics, Forsyth Institute, Cambridge, MA, 2School of Medicine, University of California, Davis, CA

Objectives: The rhesus macaque serves as an animal model to study HIV infection and periodontitis. �Previously, we determined that the oral microbiome of the macaque is distinct, albeit similar, to that of the human. �The purpose of this study is to determine similarities between the subgingival plaque of younger vs older animals, who are subject to oral disease.� Methods: Bacterial DNA was isolated from subgingival plaque from 5 young animals (<5 years) and 4 older animals (>12 years), and PCR-amplified using broad range 16S rDNA bacterial primers. �Full-sized amplicons of about 1500 bp were cloned and subsequently sequenced to generate phylogenetic trees for species identification. Results: Based on the analysis of 900 clones, there were 48 predominant species, of which 27 were unique to the macaque.� The remaining species were identified as �human� species.� The most commonly-detected species found in subgingival plaque were Abiotrophia defectiva, Gemella morbillorum �(macaque specific), Streptococcus mitis bv2, and a Lachnospiraceae phylotype.�� �Species of Actinomyces, Veillonella, Eubacterium and related spp., and Prevotella, typically detected in humans, were notably absent in younger monkeys, but present in the older monkeys.� �Conclusions: �The oral microbiome of the subgingival plaque of the rhesus macaque is distinct from that of humans.� Although some macaque species are also found in humans, most are unique to the monkey. �Bacterial profiles of subgingival plaque are age-dependent, with more �typical� periodontal pathogens present in older monkeys.� These findings help to validate using the macaque monkey as a model for human disease. Supported by DE020025.


This abstract is based on research that was funded entirely or partially by an outside source: DE020025

Keywords: Animal, Microbiology, Periodontal disease and Periodontal organisms