1330 High Biocompatibility of Endosolv R on HPDL Cells

Saturday, March 24, 2012: 9:45 a.m. - 11 a.m.
Presentation Type: Poster Session
A. CHUANG1, J. GOODIN1, J. HWANG2, and J. MCPHERSON1, 1Eisenhower Army Medical Center, Fort Gordon, GA, 2Endodontics, Eisenhower Army Medical Center, Fort Gordon, GA
Objectives: Chloroform is recognized as the most efficient solvent for endodontic retreatment, while EndoSolv R is designed to remove phenolic resin fillers.In this study we evaluated the biocompatibility of EndoSolv R on human periodontal ligament fibroblasts (HPDL) and compared with that of chloroform.


HPDL cells were isolated and cultured from healthy patients’ extracted teeth. The cells were maintained in BioWhittaker UltrCULTURE medium containing FBS and antibiotics. The concentrations of EndoSolv R and chloroform used to treat HPDL cells were 0 (control group), 5, 10, 20, 30 or 40 mM, exposed for 30 or 60 min per day. Cells were stained with crystal violet on day 1, 2 and 4, and then dissolved in 10% acetic acid solution. The absorbance was measured at 560 nm spectrophotometrically.

Results: The cell viability with chloroform at 5-20 mM was 80 % to 120% at all treatment times while at 30-40 mM was 38-57% on day 1; 15-35% on day 2; 12-16% on day 4. The cell viability with EndoSolv R stayed at 80-97% at all concentrations and treatment times.


 1) The biocompatibility profiles of EndoSolv R and chloroform on HPDL cells appeared to be very different; 2) With chloroform treatment at 5-10 mM, 10-20% cell growth was enhanced on day 1; on day 4 at 30-40 mM, cell viability decreased significantly (12-33%);  3) EndoSolv R showed high biocompatibility under our experimental  parameters.

Keywords: Biocompatibility, Cell culture and Fibroblasts