High Biocompatibility of Endosolv R on HPDL Cells

Objectives: Chloroform is recognized as the most efficient solvent for endodontic retreatment, while EndoSolv R is designed to remove phenolic resin fillers.In this study we evaluated the biocompatibility of EndoSolv R on human periodontal ligament fibroblasts (HPDL) and compared with that of chloroform.

Methods:

HPDL cells were isolated and cultured from healthy patients’ extracted teeth. The cells were maintained in BioWhittaker UltrCULTURE medium containing FBS and antibiotics. The concentrations of EndoSolv R and chloroform used to treat HPDL cells were 0 (control group), 5, 10, 20, 30 or 40 mM, exposed for 30 or 60 min per day. Cells were stained with crystal violet on day 1, 2 and 4, and then dissolved in 10% acetic acid solution. The absorbance was measured at 560 nm spectrophotometrically.

Results: The cell viability with chloroform at 5-20 mM was 80 % to 120% at all treatment times while at 30-40 mM was 38-57% on day 1; 15-35% on day 2; 12-16% on day 4. The cell viability with EndoSolv R stayed at 80-97% at all concentrations and treatment times.

Conclusions:

 1) The biocompatibility profiles of EndoSolv R and chloroform on HPDL cells appeared to be very different; 2) With chloroform treatment at 5-10 mM, 10-20% cell growth was enhanced on day 1; on day 4 at 30-40 mM, cell viability decreased significantly (12-33%);  3) EndoSolv R showed high biocompatibility under our experimental  parameters.