Method: After Jurkats were co-cultured with Fn for four hours, we assessed the expression of PGRP-S on Jurkats via flow cytometry (MoFlo Systems). After co-culturing Fn with Jurkats overnight with monoclonal antibodies to PGRP-S, the levels of Jurkat apoptosis was assessed via caspase assay according to manufacturer instructions (Promega Caspase-Glo 3/7 Assay Kit). ANOVA with post-hoc Tukey’s test was used for statistical analysis at p<0.05.
Result: A significant increase in PGRP-S expression was found on Jurkat-cells upon co-culture with Fn. There was a significant dose dependent decrease in Jurkat-cell apoptosis upon co-culture with Fn in the presence of monoclonal anti-PGRP-S antibodies.
Conclusion: The results indicate that PGRP-S has a direct role in Fusobacterium nucleatum’s ability to induce apoptotic cell death in Jurkat T cells. Further studies are needed to elucidate the signaling pathway involved in this process.
Keywords: Apoptosis, Bacterial, Endodontics, Periodontal disease and Pulpal disease