Method: Double KO mice were created by crossing both heterozygous periostin and sclerostin mice. Their phenotypic changes were examined at 3-month of age. These mice were analyzed to study the osteocyte changes and the effect of blocking SOST on alveolar bone and PDL. Radiographs, MicroCT, histology, SEM, and immuno-histochemistry were utilized for phenotypic analyses.
Result: Irregular cell shape and a sharp reduction in mineral content surrounding osteocyte body and dendrites were observed in periostin KO mice. There was a higher bone resorption, leading to alveolar bone loss in the periostin KO mice. Bone anabolic effects with significant increases in alveolar bone volume was observed in periostin KO mice by knocking out the sclerostin gene. Similarly, blocking SOST greatly improved osteocyte phenotype and decreased bone resorption in periostin KO mice.
Conclusion: Our data revealed a high bone resorption with abnormal osteocytes in periostin KO mice, and that deleting SOST completely reverse bone loss in periostin KO mice. This study suggests that deletion of sclerostin protects against alveolar bone loss in periostin KO mice.
Keywords: Bone repair, Periodontal disease and Periostin
See more of: AADR/Johnson & Johnson Oral Health Products Hatton Awards