834 Fluoride release and recharge of antibacterial and fluoride-releasing sealants

Friday, March 23, 2012: 2 p.m. - 3:15 p.m.
Presentation Type: Poster Session
E. HENDER, Y. WANG, J. ZHANG, J. HAGAN, and X. XU, Comprehensive Dentistry and Biomaterials, Louisiana State University, New Orleans, LA

Sealants with higher fluoride release and recharge capability, and additional antibacterial effect and bioactivity may have better ability to prevent primary and secondary caries. Objectives: The objective of this in vitro study is to test and compare the fluoride release and recharge capabilities of experimental antibacterial fluoride –releasing sealants and commercial fluoride-releasing sealants. Methods: Two experimental antibacterial fluoride-releasing sealants (Exp-1 and Exp-2) were formulated using a synthesized antibacterial fluoride-releasing monomer (16.13%), other dental monomers (48.87%) and fluoride-releasing filler (Caulk/Dentsply) (35%). Exp-2 also contains 15% NovaMin bioactive glass particles (GlaxoSmithKline) replacing 15% of the fluoride-releasing filler. Two commercial fluoride-releasing sealants FluroShield (FS) (Caulk/Dentsply) and Clinpro (CP) (3M-ESPE) were also included.  The disk specimens (5.0 mm diameter, 1.2 mm thick, n=5) were prepared and immersed in 2.0mL deionized water at 37oC.  The fluoride concentration of the solution (1.8 mL immersion solution plus 0.3 mL TISAB III) was measured daily using ion-selective electrode (Thermo-Orion 96-09) and 720 pH/ISE meter (Thermo-Orion) for 14 days with daily replenish of the solution. The specimens were then recharged with Neutra-Foam (2.0% NaF) (Oral –B) for 1 min and rinsed with running deionized water for 30s. Fluoride release from recharged samples was measured daily for 5 days and recharge was repeated three times. The data were analyzed using ANOVA and Tukey's Honestly Significant Differences test.

Results: Table 1 Cumulative fluoride release in 14 day and 3days after recharge (Mean±SD)


Cumulative F release n 14 days (µg/cm2)

F release in 3days after recharge (µg/cm2)













Groups with different superscript letters have significant difference (p<0.05)

Conclusions:   the experimental antibacterial fluoride-releasing sealants have significantly higher fluoride release and recharge capabilities than commercial fluoride-releasing sealants. (Supported by NIH/NIDCR grant 5R01DE019203-03).


This abstract is based on research that was funded entirely or partially by an outside source: NIH/NIDCR grant 5R01DE019203-03

Keywords: Fluoride and Sealants