816 Biocompatibility of five synthesized dental monomers

Friday, March 23, 2012: 2 p.m. - 3:15 p.m.
Presentation Type: Poster Session
Y. WANG, T. LALLIER, J. ZHANG, Y. FAN, and X. XU, Comprehensive Dentistry and Biomaterials, Louisiana State University, New Orleans, LA

Objectives: To study the cytotoxicity of five novel, synthesized dental monomers. �

Methods: Five functional dental monomers have been synthesized: self-etching acid monomer (M-1), chelating dimethacrylate monomer (M-2), antibacterial monomer (M-3), fluoride-exchange monomer (M-4), and antibacterial fluoride-exchange monomer (M-5). BisGMA was included as control. �Human gingival fibroblasts were obtained from extracted molars from patients with healthy gingiva following informed consent as prescribed in an approved IRB protocol. �Gingival fibroblasts were maintained in MEMα containing 10% fetal calf serum (FCS) and 200 units/ml penicillin and 200 μg/ml streptomycin. �Cells were grown in 48-well plates for 24 hours prior to exposure to the monomers. �The growth media containing 0.1%DMSO were supplemented with 10-4M, 10-5M, 10-6M, and 10-7M of five synthesized monomers were added to cells for 24hrs. �BisGMA served as a positive control for cytotoxicity.� Cell survival was visualized using a fluorescent esterase substrate (Calcein-AM) and a Nikon TE2000 inverted fluorescent microscope.� Cell survival was quantified using a BioTek Synergy 2 fluorescent multi-well plate reader.� The data were analyzed using ANOVA and Tukey post hoc tests.

Results:

Monomers M-1, M-2 and M-4 show little to no detectable cytotoxicity, with cell retaining their normal, elongated morphology. �M-5 has a cytotoxicity similar to BisGMA (p>0.05).� M-3 was slightly cytotoxic, but significantly less than BisGMA (p<0.05). �

Survival rate of fibroblast cells under different monomers (%) (Mean �SD)

 

Monomers

Concentration

M-1

M-2

M-3

M-4

M-5

BisGMA

0% (Control)

100�17

100�5

100�12

100�7

100�8

100�9

0.5% DMSO

87�17

108�12

92�17

99�16

102�11

103�13

10-7

78�19

105�18

107�17

103�5

107�11

104�10

10-6

74�21

98�16

105�20

103�11

99�14

102�9

10-5

97�9

99�10

99�9

98�11

96�4

96�10

10-4

99�13

93�9

37�14

76�7

11�1

13�2

Conclusions: �All of the synthesized monomers are acceptable for dental applications. �In general, those monomers designed to be antibacterial displayed greater cytotoxicity.� Supported by NIH/NIDCR grant 5R01DE019203-3.

This abstract is based on research that was funded entirely or partially by an outside source: NIH/NIDCR grant 5R01DE019203-3

Keywords: Biocompatibility and monomer