Objectives: To study the cytotoxicity of five novel, synthesized dental monomers. �
Methods: Five functional dental monomers have been synthesized: self-etching acid monomer (M-1), chelating dimethacrylate monomer (M-2), antibacterial monomer (M-3), fluoride-exchange monomer (M-4), and antibacterial fluoride-exchange monomer (M-5). BisGMA was included as control. �Human gingival fibroblasts were obtained from extracted molars from patients with healthy gingiva following informed consent as prescribed in an approved IRB protocol. �Gingival fibroblasts were maintained in MEMα containing 10% fetal calf serum (FCS) and 200 units/ml penicillin and 200 μg/ml streptomycin. �Cells were grown in 48-well plates for 24 hours prior to exposure to the monomers. �The growth media containing 0.1%DMSO were supplemented with 10-4M, 10-5M, 10-6M, and 10-7M of five synthesized monomers were added to cells for 24hrs. �BisGMA served as a positive control for cytotoxicity.� Cell survival was visualized using a fluorescent esterase substrate (Calcein-AM) and a Nikon TE2000 inverted fluorescent microscope.� Cell survival was quantified using a BioTek Synergy 2 fluorescent multi-well plate reader.� The data were analyzed using ANOVA and Tukey post hoc tests.
Results:
Monomers M-1, M-2 and M-4 show little to no detectable cytotoxicity, with cell retaining their normal, elongated morphology. �M-5 has a cytotoxicity similar to BisGMA (p>0.05).� M-3 was slightly cytotoxic, but significantly less than BisGMA (p<0.05). �
Survival rate of fibroblast cells under different monomers (%) (Mean �SD)
| Monomers | |||||
Concentration | M-1 | M-2 | M-3 | M-4 | M-5 | BisGMA |
0% (Control) | 100�17 | 100�5 | 100�12 | 100�7 | 100�8 | 100�9 |
0.5% DMSO | 87�17 | 108�12 | 92�17 | 99�16 | 102�11 | 103�13 |
10-7 | 78�19 | 105�18 | 107�17 | 103�5 | 107�11 | 104�10 |
10-6 | 74�21 | 98�16 | 105�20 | 103�11 | 99�14 | 102�9 |
10-5 | 97�9 | 99�10 | 99�9 | 98�11 | 96�4 | 96�10 |
10-4 | 99�13 | 93�9 | 37�14 | 76�7 | 11�1 | 13�2 |
Conclusions: �All of the synthesized monomers are acceptable for dental applications. �In general, those monomers designed to be antibacterial displayed greater cytotoxicity.� Supported by NIH/NIDCR grant 5R01DE019203-3.
Keywords: Biocompatibility and monomer