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Objective: To compare the fluoride response of HE and BE caries lesions in an established pH-cycling caries model.
Methods: Caries-like lesions were formed in HE and BE (lactic acid solution-LA). Test products were: 0/250/1100ppm F NaF/silica formulation 1, 1100ppm F NaF/silica formulation 2, 1100ppm F MFP/calcium carbonate. The daily cycling regimen comprised: 4x1min dentifrice slurry treatments; 1x4h LA challenge and remineralization in a 50:50-mixture of pooled human/artificial saliva. After 20days, Vickers surface microhardness (VHN) was measured (REM=change from baseline), EFU determined (microdrill), specimens were challenged with LA and VHN measured again (DEM). Data were analyzed using two-way ANOVA (alpha=0.05; factors: enamel, treatment).
Results: Both enamel type and treatment as well as their interaction affected REM and DEM. EFU was only affected by treatment. Table shows means (SEM).
enamel | Treatment | REM | DEM | EFU | |||
human | 0F-NaF1 | 0(2) | C | -8(2) | D | 124(191) | D |
250F-NaF1 | 11(2) | B | 7(2) | C | 1415(191) | C | |
1100F-NaF1 | 45(2) | A | 35(2) | B | 4078(191) | B | |
1100F-NaF2 | 50(3) | A | 44(2) | A | 4776(196) | A | |
1100F-MFP | 7(2) | BC | 4(2) | C | 1344(196) | C | |
bovine | 0F-NaF1 | -2(2) | d | -6(2) | c | 122(196) | c |
250F-NaF1 | 13(3) | c | 9(2) | b | 1314(191) | b | |
1100F-NaF1 | 74(2) | b | 58(2) | a | 3826(196) | a | |
1100F-NaF2 | 81(2) | a | 64(2) | a | 4267(191) | a | |
1100F-MFP | 10(2) | c | 7(2) | b | 1143(196) | b | |
Significant differences between treatments within enamel types are highlighted by letters, differences between enamel types within treatments in bold.
HE and BE showed a good F dose-response and correlated well. BE showed more remineralization than HE, but only at elevated [F]. There were good correlations between fluoride concentration (0/250/1100ppm F-NaF1;[F]) vs. REM, [F] vs. EFU and REM vs. EFU, regardless of enamel type.
Conclusions: Human and bovine enamel showed a similar fluoride response, but of different magnitudes with bovine enamel being more reactive to fluoride.
This study was in part supported by GlaxoSmithKline.
Keywords: Caries, Demineralization, Enamel, Fluoride and Remineralization
See more of: Cariology Research - Demin/Remineralization