1313 Twenty-four hour microtensile bond strength of four adhesives to dentin

Saturday, March 24, 2012: 9:45 a.m. - 11 a.m.
Presentation Type: Poster Session
S. KYSON, D. CAKIR, P. BECK, L. RAMP, J. BURGESS, and M. KYSON, University of Alabama at Birmingham, Birmingham, AL

Objectives: To measure and compare the 24-hour microtensile bond strength of four self etching adhesives (Table) to dentin. Methods: Freshly extracted human molars were wet-ground with a series of silica carbide paper ending with 320 grit to obtain flat intermediate level dentin surfaces and a uniform smear layer using a rotational polishing device (Buehler/Evanston/IL). The teeth were randomly assigned to one of four groups and the adhesives for each group was applied following manufacturers' instructions. Resin composite (Z100/3M ESPE) was placed in 2mm increments over the cured adhesive and light-cured (Elipar S10/3M ESPE/1100mW/cm) until a 10mm thickness was obtained. Specimens were stored (distilled water/37C/24h) then sectioned into approximately 1x1mm thick beams comprised of dentin, adhesive and composite build-up using a slow-speed cutting device (Isomet) and a 0.004 thick blade with water-cooling. Before testing, the dimensions of each beam was measured with a digital micrometer to calculate the bonded surface area at the adhesive-dentin interface. The beams were attached horizontally to the microtensile testing machine fixture, attached to Instron (5565) and loaded in tension until failure using a 1mm/min cross head speed. Tested samples were examined with a digital microscope (Keyence/VHX-600) at 100X to determine failure mode. Failure mode was classified as adhesive to the dentin or composite, cohesive to the dentin or composite or mixed (a combination of adhesive and cohesive). Data was analyzed with ANOVA and Tukey/Kramer tests (p=0.05).

Results: (MeanSD).


Bond Strength


MTB 200/Kuraray


iBond/Heraeus Kulzer


OptiBond All-In-One/Kerr


Xeno IV/L.D. Caulk


Conclusions: OptiBond All-In-One and MTB 200 had higher microtensile bond strengths to dentin compared to iBond (p=0.0004). No other differences were found between the tested materials. Supported in part by a grant from Kuraray.


Keywords: Adhesion, Dental materials, Dentin bonding agents and Teeth