1421 Effects of Different Remineralization Methods on Artificial Early Enamel Lesions

Saturday, March 24, 2012: 9:45 a.m. - 11 a.m.
Presentation Type: Poster Session
Z.B. KUTUK, Dept. of Restorative Dentistry, Hacettepe University, Faculty of Dentistry, Ankara, Turkey, E. FIRAT, Dept. of Restorative Dentistry, Hacettepe University, Ankara, Turkey, and S. GURGAN, Dept.Restorative Dentistry, Hacettepe University, Ankara, Turkey
Objective: To assess the remineralization effects of ozone, sodium fluoride, resin infiltration and caseinphosphopeptide-amorphous calciumphosphate(CPP-ACP) on artificially demineralized lesions created in enamel.

Method: 80 caries-free mandibular third molars were embedded in the self-cured acrylic resin and buccal enamel surfaces were grounded with 1000 grid silicon-carbide papers. Enamel surfaces were coated with nail varnish leaving 8x6mm windows for exposure to demineralization/remineralization cycles. To compose artificial early enamel lesions, demineralization solution was prepared(pH: 4.8) and each tooth was kept in this solution  for 72h at 37°C. After lesion formation, teeth were randomly divided in five groups(N=16):(1)Artificial saliva(negative control group)(pH:7.0), (2)Ozone (Ozonytron®X/Mymed,Munich,Germany), (3) NaF varnish(Colgate Duraphat Varnish/Colgate-Palmolive Company,New York,USA)(positive control group), (4)Resin infiltration system(Icon®/DMG,Hamburg,Germany), (5)CPP-ACP creme(Tooth Mousse/GC Corp.,Tokyo,Japan). Subsequently, all the specimens were subjected to pH cycling for 10 days to simulate the oral demineralization/remineralization conditions. The enamel surface microhardness and roughness measurements were determined at the baseline(T0), after demineralization(T1) and after pH-cycling(T2). The data were statistically analyzed with Kolmogorov-Smirnov test(p<0.05), Kruskal-Wallis(p<0.001), Friedman-test(p<0.001) and Two-Way ANOVA(p<0.05) using SPSS PASW statistics 15.0 software. 

Result: No significant differences were observed among the groups at T0 and T1both for microhardness and surface roughness(p>0.05). The microhardness values at T2 were higher than T1 and lower than T0 in each group(p<0.001). T2 microhardness values revealed significant differences among the groups(p<0.001). CPP-ACP creme and resin infiltration applications increased the microhardness of artificial early enamel lesions significantly more than the arificial saliva, ozone and NaF varnish did(p<0.001). In respect to surface roughness, T2 values were lower than T1 and higher than T0(p<0.05) except resin infiltration  group in which a slight increase was observed at T2(p>0.05). However, there were no significant differences at T2 surface roughness among the groups(p>0.05).

Conclusion: CCP-ACP creme and resin infiltration applications are more effective on the remineralization of  early enamel lesions in comparison to NaF varnish and ozone applications.

Keywords: Caries, Demineralization, Enamel and Remineralization