630 Digital Image Analysis for Reliable Characterization of Cleft Wound Phenotypes

Friday, March 23, 2012: 8 a.m. - 9:30 a.m.
Presentation Type: Oral Session
B. SMITH1, L.M. MORENO2, S.F. MILLER3, G. WEHBY1, and M. DUNNWALD4, 1University of Iowa, Iowa City, IA, 2Orthodontics, University of Iowa, Iowa City, IA, 3Dows Institute for Dental Research, University of Iowa, Iowa City, IA, 4Pediatrics, University of Iowa, Iowa City, IA
Cleft lip is a common birth defect with major physical and psychological impacts on affected individuals’ lives.  Satisfaction with facial appearance, which is partly affected by success/quality of surgical cleft repair, is widely reported as the single most important factor influencing psychosocial adjustment among affected individuals.  Surgical success impacts wound healing, which may be influenced by IRF6 or other clefting candidate genes.  However, understanding the genetic basis of wound healing among affected individuals requires accurate characterization of the wound healing phenotype.  Objective: To develop wound healing phenotypes measured from digital 2D and 3D images of cleft lip wounds for genotype-phenotype correlation studies.  Methods:  We used 3-D images of 68 surgically repaired unilateral cleft lip individuals captured using the 3DMD image system.  Size and RGB color histogram data of affected and control regions of the philtrum and upper lip were acquired using 3DMD and Image J software.  Reliability was determined from repeated measurements of 15 images by t-test and Interclass Correlation Coefficient (ICC) analysis.  Pearson correlation was used to analyze color similarity of several non-affected control points of the philtrum.  We compared the color of affected versus unaffected control areas using t-test analysis.  Results: Our method demonstrated high repeatability from ICC scores greater than 8.0 for all repeated area measurements except total unaffected area of the lip.  The color between philtrum controls was significantly similar based on Pearson correlation (p<0.001).  T-test analysis comparing color of the philtrum wound with the contralateral unaffected philtrum control (the most relevant control region) showed a significant color difference (p ranging from 1.20-05 to 1.95-14).  Conclusions: Our study establishes a new reliable method to characterize the phenotype of labial wounds in individuals with oral clefts. Correlations between genetic variants in IRF6 and other candidate genes and the wound phenotypes are currently being evaluated and will be presented.
This abstract is based on research that was funded entirely or partially by an outside source: Data collection was funded by CDC grant 5R01DD000295 Partial support for this project was provided by R03-AR055313 and T32 DEO14678-09

Keywords: Cleft lip-palate, Genetics and Wound healing
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