1258 CD49f is a putative odontogenic epithelial stem cell marker

Saturday, March 24, 2012: 9:45 a.m. - 11 a.m.
Presentation Type: Poster Session
J.Y.F. CHANG, Biomedical Sciences, Baylor College of Dentistry, Houston, TX, R. D'SOUZA, Biomedical Science, Baylor College of Dentistry, Dallas, TX, and F. WANG, Texas A&M Health Science Center, Houston, TX
Objective: Elucidation of the cellular and molecular mechanisms that maintain mouse incisor unlimited growing potential is of broad interest and paramount for the future regenerative therapy. However, analysis of the properties and regulation of odontogenic epithelial stem cells (OESC) has been limited by a lack of well-accepted stem cell marker. The purpose of our study was to identify a stem cell surface marker for isolation and enrichment stem cell derived from mouse incisor cervical loops (CL) where OESC are believed to reside.

Method: We examined the RNA expression profiles in both mouse incisor CL tissue and sphere cells from our newly established OESC culture system. High expression of Sca-1, CD49f, and CD44 were found in the spheres compare to the CL tissue. Fluorescence-activated cell sorting (FACS) analyses of mouse incisor CL and OESC sphere cells with Sca-1, CD49f, and CD44 were also performed. Sphere forming assays with Sca-1, CD49f, and CD44 FACS sorted cells were used for evaluate the self-renewal and differentiation ability of each marker. Immunofluorescent studies were also performed to examine the location of these markers.

Result: FACS analyses of mouse incisor CL and OESC sphere cells based on CD44+ or Sca1+ did not enrich the cells with high sphere-forming activities. In contrast, CD49f+ cells were able to form spheres at a significantly high ratio than CD49f- cells. We also demonstrated that CD49fBright subpopulation further enriched the sphere forming cells among the CD49f+ population. CD49f+ cells were able to self-renewal and differentiate into cytokeratin 14, amelogenin expressed, and mineral material producing cells.

Conclusion: The development of an in vitro sphere culture and identifying CD49fBright as a stem cell marker for OESC will shed light on understanding the signaling networks to regulate the stem cell maintenance, cell fate, and differentiation. 

This abstract is based on research that was funded entirely or partially by an outside source: NIH/NIDCR K08 Award DE020883, P.I. Julia Chang

Keywords: Cell culture, Teeth and stem cells
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