825 Cytotoxicity of Two Root Canal Sealers in V79 Fibroblast Cells

Friday, March 23, 2012: 2 p.m. - 3:15 p.m.
Presentation Type: Poster Session
D.B. ANDRADE, Department of Endodontics, University of Florida College of Dentistry, Gainesville, FL, T.R. OLIVEIRA, Department of Endodontics, São Paulo State University, São José dos Campos, Brazil, G.O. SILVA, UNESP, São José dos Campos, Brazil, A.L. RIBEIRO, Department of Endodontics, Sao Paulo State University, Sao Jose dos Campos, Brazil, C.H.R. CAMARGO, Operative Dentistry and Endodontics, Universidade Est. Paulista Julio Mesquita, São José dos Campos, Brazil, and S.E.A. CAMARGO, Bioscience and Buccal Diagnosis, Universidade Est. Paulista Julio Mesquita, São Jose dos Campos, Brazil
Objective: The aim of this study was to evaluate the cytotoxicity of the two components of the root canal sealer RealSeal  (R and W) and the two components of the root canal sealer EndoRez (W and Y) when put in contact with V79 fibroblast Cells in a separated or mixed form.

Method: The components were inserted into 24-well plates in a isolated or mixed forms and were immediately placed in contact with the culture medium (82.4 mm2 surface / ml) at 37 ° C with 100% humidity and 5% CO2) for 24h to prepare the original extract and the others 1:1 dilutions (1:2, 1:4, 1:8, 1:16, 1:32). The extracts were applied to culture of V79 fibroblast cells and cell viability was measured by MTT assay using spectrophotometer. The data were analyzed by the U-Mann-Whitney test (α = 0.05) (SPSS / PC +, version 11.5 SPSS, Chicago, IL, USA) and Dunn (5%).

Result: The RealSeal sealer showed to be most cytotoxic when compared with the sealer EndoRez, reducing cell survival in all dilutions. The EndoRez sealer promoted cell survival of 66.8%, 78.2% and 84.3% respectively in dilutions of 1:8, 1:16 and 1:32. For the separate components, it was observed that the component R of the RealSeal presented a slightly less cytotoxic than the W component, with up to 75% cell survival in the dilution 1:32. As for the separate components of EndoRez, the component W presented a cell survival of 71.7% in the 1:32 dilution, while the Y component maintained cell survival averaged 39% since the original extract 1:1 to the 1:32 dilution.

Conclusion: The sealer RealSeal both, with mixed or separate components, showed to be more cytotoxic than the EndoRez sealer in all forms.

This abstract is based on research that was funded entirely or partially by an outside source: FAPESP

Keywords: Cell culture, Endodontics, Fibroblasts and Root canal fillings
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