Core# to Oligo Sequence Rosetta Page

Mutagenic Primers

Core# Nucleotide Sequence Receptor Mutant(s) Generated Alias Strand Complemented General Comments Receptor Region
5535F 5'-GGA ATC GTG ATC AGC GCC CAG ATG ATG ACG TAG ATG-3' rMORK303W GGB-1-OLIGO-1 Sense   Top TM-6
5536F 5'-GGA ATC GTG ATC AGC GCT TGG ATG ATG ACG TAG ATG-3' rMOR K303Q GGB-1-OLIGO-2 Sense   Top TM-6
5537F 5'-GGA ATC GTG ATC AGC GCT TCG ATG ATG ACG TAG ATG-3' rMOR K303E GGB-1-OLIGO-3 Sense   Top TM-6
5538F 5'-GGA ATC GTG ATC AGC GCT (A/C)TG ATG ATG ACG TAG ATG-3' rMOR K303R;K303I GGB-1-OLIGO-4 Sense Degenerate Oligo Top TM-6
5539F 5'-GCA ATG CAG AAG TGC TTG GAA ACC GTC TG-3' rMOR W318K GGB-1-OLIGO-5 Sense   Top TM-7
5540F 5'-GCA ATG CAG AAG TGC CGG GAA ACC GTC TG-3' rMOR W318R GGB-1-OLIGO-6 Sense   Top TM-7
5541F 5'-GCA ATG CAG AAG TGC AAG GAA ACC GTC TG-3' rMOR W318L GGB-1-OLIGO-7 Sense   Top TM-7
6645H 5'-GGA ATC GTG ATC AGC GCC CTG ATG ATG ACG TAG ATG-3' rMOR K303R mu_K303R Sense   Top TM-6

Selection Primers

6465H
Core# Alias Nucleotide Sequence Construct Site Removed Strand Complemented Comments
? ScaI Primer 5'-GAC TGG TGA GTA TTC AAC CAA GTC-3' m4B-CMV ScaI (3938-3943) Sense Note #1
6464H GB_Stu>Sca 5'-CCA TGG TGC TGA AGT ACT GCT GCG GAC TAG G-3' m4B-CMV StuI (1123-1128) Sense Note #3
GB_Sca>Stu 5'-CCA TGG TGC TGA AGG CCT GCT GCG GAC TAG G-3' m4B-CMV ScaI (1123-1128) Sense Note #3

Sequencing Primers

6463H 6462H 6461H 9330H
Core# Alias Nucleotide Sequence Sequence Position Construct Strand complemented
GB_mu_seq1 5'-GGT TCC GAA GGG CCA TGT TCC-3' 1527-1547 m4B Sense
GB_mu_seq2 5'-GCA GGT TCT CCC AGT ACC AGG-3' 1810-1830 m4B Sense
GB_mu_seq3 5'-GGA TTC AGG CAG CTG TTC G-3' 2116-2134 m4B Sense
GB2-100 5'-GCC AGG AGC CAG GTG CTG GGG-3' 1204-1224 m4B Sense
? pm-il3 5'-GCC CGA TAG CAT GCG AAC GC-3' 1918-1937 m4B Sense
Rosetta Stone Picture Please email me if you find any errors, obvious omissions or you just want to make a comment. I want this to be as accurate and complete as possible.
Last Changed 13MAY1998
Gregg Bonner