.. _E2F3: E2F3 ^^^^ .. contents:: :local: General Information ******************* :Full gene name: E2F transcription factor 3 :Entrez Gene ID: 1871 :Location: 6p22 :Synonyms: E2F-3 :Type: protein-coding User SNPs ********* SNPs given by the user that are near or inside this gene: +-----------+---------------+------------+ | SNP | Distance (bp) | Direction | +===========+===============+============+ | rs7754840 | 167305 | downstream | +-----------+---------------+------------+ .. _E2F3 Gene summary: NCBI Summary ************ The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011] .. _OMIM ID 600427 : http://omim.org/entry/600427 .. _E2F3 OMIM Text: OMIM **** :OMIM ID: `OMIM ID 600427 `_ .. _E2F3 Phenotype: NCBI Phenotypes *************** No phenotypes found linked to this gene. .. _E2F3 GO Term: Gene Ontology ************* * mitotic cell cycle * transcription initiation from RNA polymerase II promoter * G1 phase of mitotic cell cycle * nucleoplasm * protein binding * G2 phase of mitotic cell cycle * sequence-specific DNA binding transcription factor activity * positive regulation of transcription, DNA-dependent * regulation of transcription, DNA-dependent * transcription factor complex * positive regulation of cell proliferation * core promoter binding .. _E2F3 Pathway: KEGG Pathways ************* * `Prostate cancer `_ * `Melanoma `_ * `Glioma `_ * `Bladder cancer `_ * `Small cell lung cancer `_ * `Non-small cell lung cancer `_ * `Pathways in cancer `_ * `HTLV-I infection `_ * `Pancreatic cancer `_ * `Chronic myeloid leukemia `_ * `Cell cycle `_ .. _E2F3 GeneRIF: GeneRIFs ******** * Affinity Capture-MS [`PMID 16147992`_] * E2F3 (E2F-3) interacts with the BIRC5 (survivin) promoter. [`PMID 15271987`_] * just as E2F3, HELLS is overexpressed in human tumours including prostate cancer, indicating that either factor may contribute to the malignant progression of tumours [`PMID 22157815`_] * Affinity Capture-Western [`PMID 22157815`_] * Affinity Capture-Western [`PMID 22157815`_] * Affinity Capture-Western [`PMID 22157815`_] * Affinity Capture-MS; Affinity Capture-Western; Reconstituted Complex [`PMID 22157815`_] * Affinity Capture-Western [`PMID 22157815`_] * E2F3 interacts with the B-myb promoter region. [`PMID 10766737`_] * E2F3 interacts with the Cdc25A promoter. [`PMID 10766737`_] * E2F3 interacts with the Cdc6 promoter. [`PMID 10766737`_] * E2F3 interacts with the Cdc2 promoter. [`PMID 10766737`_] * Data show that E2F is likely a central coordinator of multiple responses that culminate in regulation of EBP1 gene expression, and which may vary depending on cell type and context. [`PMID 21085677`_] * E2F3 mRNA and protein are overexpressed in retinoblastoma. [`PMID 16180235`_] * E2F3 interacts with EAPP. [`PMID 15716352`_] * A significant elevation in expression of E2F3 in oral squamous cell carcinoma cells was seen in carbon and neon-irradiated cells. [`PMID 18514338`_] * E2F3 interacts with the MT1G promoter. [`PMID 15735762`_] * determination of interaction with RYBP and YY1 [`PMID 12411495`_] * Affinity Capture-Western; Two-hybrid [`PMID 12411495`_] * Two-hybrid [`PMID 12411495`_] * Two-hybrid [`PMID 12411495`_] * E2F3 interacts with RYBP. [`PMID 12411495`_] * E2F3 interacts with a molecule expressed by Gene FHL2. The precise molecular variant of FHL2 involved in this interaction is not specified. [`PMID 12411495`_] * the hypoxia component of ischemia may limit wound re-epithelialization by stabilizing HIF-1alpha, which induces miR-210 expression, resulting in the down-regulation of the cell-cycle regulatory protein E2F3 [`PMID 20308562`_] * E2F3 gain is associated with the late stage of retinoblastoma. [`PMID 18785023`_] * We conclude that E2F3 is frequently amplified and overexpressed in invasively growing bladder cancer (stage pT1-4). E2F3 expression appears to provide a growth advantage to tumor cells by activating cell proliferation in a subset of bladder tumors. [`PMID 15122326`_] * E2F1, E2F2, and E2F3 directly bind the promoter of the mir-17-92 cluster, activating its transcription. miR-20a from the mir-17-92 cluster in turn modulates the translation of E2F2 and E2F3, suggesting an autoregulatory feedback loop. [`PMID 17135249`_] * Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) [`PMID 21048031`_] * E2F3 interacts with the JMY promoter. [`PMID 15706352`_] * E2F3 interacts with the PPP1R13B (ASPP1) promoter. [`PMID 15706352`_] * E2F3 interacts with the TP53BP2 (ASPP2) promoter. [`PMID 15706352`_] * E2F3 interacts with the TP53INP1 promoter. [`PMID 15706352`_] * E2F3a induces apoptosis in HepG2 cells and plays important roles in regulating transcription [`PMID 20803551`_] * E2F3 levels have a critical role in modifying cellular proliferation rate in human bladder and prostate cancer. [`PMID 16909110`_] * Inactivation of the retinoblastoma pathway occurs in bladder tumors with 6p22 amplification. [`PMID 18037967`_] * Observational study of gene-disease association. (HuGE Navigator) [`PMID 19738611`_] * requirement of E2F3 for BCR/ABL leukemogenesis [`PMID 17925491`_] * Affinity Capture-Western [`PMID 16951254`_] * Genes involved in the E2F3 pathway are associated with chemotherapy sensitivity among ER- tumors. The mutant p53 signature and expression of ER-related genes were associated with lower sensitivity to chemotherapy in ER+ breast tumors only. [`PMID 18445275`_] * The Gab2 promoter region interacts with E2F3. [`PMID 15574337`_] * Expression of E2F3 was lowest in early-stage tumors and highest in metastatic tissue. Expression profiling of miRNAs in WT showed that expression of each measured member of the Oncomir-1 family was highest in WT relative to other kidney tumor subtypes [`PMID 18519660`_] * Reconstituted Complex [`PMID 8657141`_] * E2F3 interacts with the MSH2 promoter. [`PMID 11799067`_] * E2F3 interacts with the UXT chromatin. [`PMID 11799066`_] * Study demonstrates the strength of E2F3 as a potential marker for discriminating benign and malignant disease, addressing the current limitations of serum PSA measurements. [`PMID 17701752`_] * miR-203 induced cellular senescence by targeting E2F3 in human melanoma Mewo and A2058 cells [`PMID 22354972`_] * E2F-3 interacts with the E2F site. This interaction was modelled on a demonstrated interaction between E2F-3 and a synthetic dsDNA construct. [`PMID 8246996`_] * Affinity Capture-Western; Co-localization [`PMID 15014447`_] * E2F3 is overexpressed in 55-70% of squamous cell carcinomas and 79% of adenocarcinomas of the lung. In addition very high level expression of nuclear E2F3 is found in almost all small cell lung cancers analysed. [`PMID 16938365`_] * E2F3 contributes to ataxia telangiectasia mutated (ATM) kinase-dependent p53 phosphorylation and apoptosis in cells expressing E1A. [`PMID 18235226`_] * E2F3 modulates Aurora-A mRNA expression during the cell cycle. [`PMID 18776222`_] * Data report that ANCCA directly interacts with E2F1 to E2F3 in its complex with MLL and that its N terminus interacts with both the N and C termini of E2F1. [`PMID 20855524`_] * Affinity Capture-Western; Reconstituted Complex [`PMID 20855524`_] * Affinity Capture-Western; Two-hybrid [`PMID 17612494`_] * miR-34a targeting the Sirt1 and E2F3 genes could negatively regulate the resistance to 5-FU in human colorectal cancer DLD-1 cells. [`PMID 21067862`_] * these data reveal the novel ability of E2f3 to function as a master regulator of the DNA damage response. [`PMID 19917728`_] * results suggest that DEK and E2F3 are potential targets of 6p gains in retinoblastoma [`PMID 16007192`_] * In bladder cancer, the E2F3 gene is associated with overexpression of its encoded mRNA transcripts and high levels of E2F3 expression. [`PMID 14716298`_] * report confirms significant mRNA overexpression of KIF14 and E2F3 together in a large cohort of retinoblastoma tumors [`PMID 19190782`_] * miR-128 can inhibit proliferation of glioma cells through one of its targets, E2F3a. [`PMID 18810376`_] * human Pumilio homologs Pum 1 and Pum 2 repress the translation of E2F3 by binding to the E2F3 3' untranslated region (UTR) and also enhance the activity of multiple E2F3 targeting microRNAs (miRNAs) [`PMID 22345517`_] * Data suggest that the physical interaction of TFE3 and E2F3 facilitates transcriptional activation of the p68 gene and provides strong evidence for the specificity of E2F function. [`PMID 12748276`_] * Two-hybrid [`PMID 12748276`_] * Two-hybrid [`PMID 12748276`_] * Affinity Capture-Western; Reconstituted Complex; Two-hybrid [`PMID 12748276`_] * Two-hybrid [`PMID 12748276`_] * Two-hybrid [`PMID 12748276`_] * Two-hybrid [`PMID 12748276`_] * Two-hybrid [`PMID 12748276`_] * Two-hybrid [`PMID 12748276`_] .. _PMID 16147992: http://www.ncbi.nlm.nih.gov/pubmed/16147992 .. _PMID 15271987: http://www.ncbi.nlm.nih.gov/pubmed/15271987 .. _PMID 22157815: http://www.ncbi.nlm.nih.gov/pubmed/22157815 .. _PMID 10766737: http://www.ncbi.nlm.nih.gov/pubmed/10766737 .. _PMID 21085677: http://www.ncbi.nlm.nih.gov/pubmed/21085677 .. _PMID 16180235: http://www.ncbi.nlm.nih.gov/pubmed/16180235 .. _PMID 15716352: http://www.ncbi.nlm.nih.gov/pubmed/15716352 .. _PMID 18514338: http://www.ncbi.nlm.nih.gov/pubmed/18514338 .. _PMID 15735762: http://www.ncbi.nlm.nih.gov/pubmed/15735762 .. _PMID 12411495: http://www.ncbi.nlm.nih.gov/pubmed/12411495 .. _PMID 20308562: http://www.ncbi.nlm.nih.gov/pubmed/20308562 .. _PMID 18785023: http://www.ncbi.nlm.nih.gov/pubmed/18785023 .. _PMID 15122326: http://www.ncbi.nlm.nih.gov/pubmed/15122326 .. _PMID 17135249: http://www.ncbi.nlm.nih.gov/pubmed/17135249 .. _PMID 21048031: http://www.ncbi.nlm.nih.gov/pubmed/21048031 .. _PMID 15706352: http://www.ncbi.nlm.nih.gov/pubmed/15706352 .. _PMID 20803551: http://www.ncbi.nlm.nih.gov/pubmed/20803551 .. _PMID 16909110: http://www.ncbi.nlm.nih.gov/pubmed/16909110 .. _PMID 18037967: http://www.ncbi.nlm.nih.gov/pubmed/18037967 .. _PMID 19738611: http://www.ncbi.nlm.nih.gov/pubmed/19738611 .. _PMID 17925491: http://www.ncbi.nlm.nih.gov/pubmed/17925491 .. _PMID 16951254: http://www.ncbi.nlm.nih.gov/pubmed/16951254 .. _PMID 18445275: http://www.ncbi.nlm.nih.gov/pubmed/18445275 .. _PMID 15574337: http://www.ncbi.nlm.nih.gov/pubmed/15574337 .. _PMID 18519660: http://www.ncbi.nlm.nih.gov/pubmed/18519660 .. _PMID 8657141: http://www.ncbi.nlm.nih.gov/pubmed/8657141 .. _PMID 11799067: http://www.ncbi.nlm.nih.gov/pubmed/11799067 .. _PMID 11799066: http://www.ncbi.nlm.nih.gov/pubmed/11799066 .. _PMID 17701752: http://www.ncbi.nlm.nih.gov/pubmed/17701752 .. _PMID 22354972: http://www.ncbi.nlm.nih.gov/pubmed/22354972 .. _PMID 8246996: http://www.ncbi.nlm.nih.gov/pubmed/8246996 .. _PMID 15014447: http://www.ncbi.nlm.nih.gov/pubmed/15014447 .. _PMID 16938365: http://www.ncbi.nlm.nih.gov/pubmed/16938365 .. _PMID 18235226: http://www.ncbi.nlm.nih.gov/pubmed/18235226 .. _PMID 18776222: http://www.ncbi.nlm.nih.gov/pubmed/18776222 .. _PMID 20855524: http://www.ncbi.nlm.nih.gov/pubmed/20855524 .. _PMID 17612494: http://www.ncbi.nlm.nih.gov/pubmed/17612494 .. _PMID 21067862: http://www.ncbi.nlm.nih.gov/pubmed/21067862 .. _PMID 19917728: http://www.ncbi.nlm.nih.gov/pubmed/19917728 .. _PMID 16007192: http://www.ncbi.nlm.nih.gov/pubmed/16007192 .. _PMID 14716298: http://www.ncbi.nlm.nih.gov/pubmed/14716298 .. _PMID 19190782: http://www.ncbi.nlm.nih.gov/pubmed/19190782 .. _PMID 18810376: http://www.ncbi.nlm.nih.gov/pubmed/18810376 .. _PMID 22345517: http://www.ncbi.nlm.nih.gov/pubmed/22345517 .. _PMID 12748276: http://www.ncbi.nlm.nih.gov/pubmed/12748276 .. _E2F3 Pubmed: PubMed Articles *************** *Recent articles:* * Noguchi S et al. "Anti-oncogenic microRNA-203 induces senescence by targeting E2F3 protein in human melanoma cells." J Biol Chem. 2012 Apr 6;287(15):11769-77. `PMID 22354972`_ * Miles WO et al. "Pumilio facilitates miRNA regulation of the E2F3 oncogene." Genes Dev. 2012 Feb 15;26(4):356-68. `PMID 22345517`_ * von Eyss B et al. "The SNF2-like helicase HELLS mediates E2F3-dependent transcription and cellular transformation." EMBO J. 2011 Dec 13;31(4):972-85. `PMID 22157815`_ * Casabonne D et al. "Single nucleotide polymorphisms of matrix metalloproteinase 9 (MMP9) and tumor protein 73 (TP73) interact with Epstein-Barr virus in chronic lymphocytic leukemia: results from the European case-control study EpiLymph." Haematologica. 2011 Feb;96(2):323-7. `PMID 21048031`_ * Akao Y et al. "Dysregulation of microRNA-34a expression causes drug-resistance to 5-FU in human colon cancer DLD-1 cells." Cancer Lett. 2011 Jan 28;300(2):197-204. `PMID 21067862`_ * Li W et al. "Characterization of E2F3a function in HepG2 liver cancer cells." J Cell Biochem. 2010 Dec 1;111(5):1244-51. `PMID 20803551`_ * Judah D et al. "EBP1 is a novel E2F target gene regulated by transforming growth factor-β." PLoS One. 2010 Nov 10;5(11):e13941. `PMID 21085677`_ * Revenko AS et al. "Chromatin loading of E2F-MLL complex by cancer-associated coregulator ANCCA via reading a specific histone mark." Mol Cell Biol. 2010 Nov;30(22):5260-72. `PMID 20855524`_ * Biswas S et al. "Hypoxia inducible microRNA 210 attenuates keratinocyte proliferation and impairs closure in a murine model of ischemic wounds." Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6976-81. `PMID 20308562`_ * Martinez LA et al. "E2F3 is a mediator of DNA damage-induced apoptosis." Mol Cell Biol. 2010 Jan;30(2):524-36. `PMID 19917728`_ .. _PMID 19917728: http://www.ncbi.nlm.nih.gov/pubmed/19917728 .. _PMID 20308562: http://www.ncbi.nlm.nih.gov/pubmed/20308562 .. _PMID 20855524: http://www.ncbi.nlm.nih.gov/pubmed/20855524 .. _PMID 21067862: http://www.ncbi.nlm.nih.gov/pubmed/21067862 .. _PMID 22157815: http://www.ncbi.nlm.nih.gov/pubmed/22157815 .. _PMID 21048031: http://www.ncbi.nlm.nih.gov/pubmed/21048031 .. _PMID 21085677: http://www.ncbi.nlm.nih.gov/pubmed/21085677 .. _PMID 20803551: http://www.ncbi.nlm.nih.gov/pubmed/20803551 .. _PMID 22354972: http://www.ncbi.nlm.nih.gov/pubmed/22354972 .. _PMID 22345517: http://www.ncbi.nlm.nih.gov/pubmed/22345517 *Top Pubmed articles linked to gene E2F3 matching any search term:* * Ignatiadis M et al. "Gene modules and response to neoadjuvant chemotherapy in breast cancer subtypes: a pooled analysis." J Clin Oncol. 2012 Jun 1;30(16):1996-2004. `PMID 22508827`_ * Real S et al. "E2F1 regulates cellular growth by mTORC1 signaling." PLoS One. 2011 Jan 24;6(1):e16163. `PMID 21283628`_ * von Willebrand M et al. "The tyrphostin AG1024 accelerates the degradation of phosphorylated forms of retinoblastoma protein (pRb) and restores pRb tumor suppressive function in melanoma cells." Cancer Res. 2003 Mar 15;63(6):1420-9. `PMID 12649208`_ .. _PMID 12649208: http://www.ncbi.nlm.nih.gov/pubmed/12649208 .. _PMID 21283628: http://www.ncbi.nlm.nih.gov/pubmed/21283628 .. _PMID 22508827: http://www.ncbi.nlm.nih.gov/pubmed/22508827