.. _IDH3G: IDH3G ^^^^^ .. contents:: :local: General Information ******************* :Full gene name: isocitrate dehydrogenase 3 (NAD+) gamma :Entrez Gene ID: 3421 :Location: Xq28 :Synonyms: H-IDHG :Type: protein-coding User SNPs ********* SNPs given by the user that are near or inside this gene: +-----------+---------------+------------+ | SNP | Distance (bp) | Direction | +===========+===============+============+ | rs3020789 | 158234 | downstream | +-----------+---------------+------------+ .. _IDH3G Gene summary: NCBI Summary ************ Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined. [provided by RefSeq, Jul 2008] .. _OMIM ID 300089 : http://omim.org/entry/300089 .. _IDH3G OMIM Text: OMIM **** :OMIM ID: `OMIM ID 300089 `_ .. _IDH3G Phenotype: NCBI Phenotypes *************** No phenotypes found linked to this gene. .. _IDH3G GO Term: Gene Ontology ************* * nucleolus * magnesium ion binding * NAD binding * negative regulation of growth * isocitrate dehydrogenase (NAD+) activity * carbohydrate metabolic process * mitochondrial matrix * small molecule metabolic process * 2-oxoglutarate metabolic process * mitochondrion * isocitrate metabolic process * nucleus * ATP binding * NADH metabolic process * tricarboxylic acid cycle .. _IDH3G Pathway: KEGG Pathways ************* * `Metabolic pathways `_ * `Citrate cycle (TCA cycle) `_ .. _IDH3G GeneRIF: GeneRIFs ******** * active sites of the human NAD-IDH are shared between alpha and gamma subunits and between alpha and beta subunits [`PMID 16737955`_] * Affinity Capture-MS [`PMID 17314511`_] * Aspartate-190 is a determinant of IDH gamma subunit affinity for the manganese (MnII) ion, as well as for nicotinamide-adenine dinucleotide (NAD), but is not directly required for the catalytic reaction. [`PMID 17432878`_] .. _PMID 16737955: http://www.ncbi.nlm.nih.gov/pubmed/16737955 .. _PMID 17314511: http://www.ncbi.nlm.nih.gov/pubmed/17314511 .. _PMID 17432878: http://www.ncbi.nlm.nih.gov/pubmed/17432878 .. _IDH3G Pubmed: PubMed Articles *************** *Recent articles:* * Barbe L et al. "Toward a confocal subcellular atlas of the human proteome." Mol Cell Proteomics. 2008 Mar;7(3):499-508. `PMID 18029348`_ * Bzymek KP et al. "Role of alpha-Asp181, beta-Asp192, and gamma-Asp190 in the distinctive subunits of human NAD-specific isocitrate dehydrogenase." Biochemistry. 2007 May 8;46(18):5391-7. `PMID 17432878`_ * Koch HB et al. "Large-scale identification of c-MYC-associated proteins using a combined TAP/MudPIT approach." Cell Cycle. 2007 Jan 15;6(2):205-17. `PMID 17314511`_ * Soundar S et al. "Identification of Mn2+-binding aspartates from alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase." J Biol Chem. 2006 Jul 28;281(30):21073-81. `PMID 16737955`_ * Mehrle A et al. "The LIFEdb database in 2006." Nucleic Acids Res. 2006 Jan 1;34(Database issue):D415-8. `PMID 16381901`_ * Gerhard DS et al. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." Genome Res. 2004 Oct;14(10B):2121-7. `PMID 15489334`_ * Wiemann S et al. "From ORFeome to biology: a functional genomics pipeline." Genome Res. 2004 Oct;14(10B):2136-44. `PMID 15489336`_ * Soundar S et al. "Evaluation by mutagenesis of the importance of 3 arginines in alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase." J Biol Chem. 2003 Dec 26;278(52):52146-53. `PMID 14555658`_ * Strausberg RL et al. "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903. `PMID 12477932`_ * Simpson JC et al. "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing." EMBO Rep. 2000 Sep;1(3):287-92. `PMID 11256614`_ .. _PMID 16737955: http://www.ncbi.nlm.nih.gov/pubmed/16737955 .. _PMID 17314511: http://www.ncbi.nlm.nih.gov/pubmed/17314511 .. _PMID 12477932: http://www.ncbi.nlm.nih.gov/pubmed/12477932 .. _PMID 14555658: http://www.ncbi.nlm.nih.gov/pubmed/14555658 .. _PMID 18029348: http://www.ncbi.nlm.nih.gov/pubmed/18029348 .. _PMID 11256614: http://www.ncbi.nlm.nih.gov/pubmed/11256614 .. _PMID 15489334: http://www.ncbi.nlm.nih.gov/pubmed/15489334 .. _PMID 17432878: http://www.ncbi.nlm.nih.gov/pubmed/17432878 .. _PMID 15489336: http://www.ncbi.nlm.nih.gov/pubmed/15489336 .. _PMID 16381901: http://www.ncbi.nlm.nih.gov/pubmed/16381901