PDE8B

General Information

Full gene name:phosphodiesterase 8B
Entrez Gene ID:8622
Location:5q13.3
Synonyms:ADSD, PPNAD3
Type:protein-coding

User SNPs

SNPs given by the user that are near or inside this gene:

SNP Distance (bp) Direction
rs4457053 81757 upstream

NCBI Summary

The protein encoded by this gene is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP. The encoded protein, which does not hydrolyze cGMP, is resistant to several PDE inhibitors. Defects in this gene are a cause of autosomal dominant striatal degeneration (ADSD). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]

OMIM

OMIM ID:`OMIM ID 603390 `_

Allelic Variants (Selected Examples)

.0001 PIGMENTED NODULAR ADRENOCORTICAL DISEASE, PRIMARY, 3

In a young girl with micronodular adrenocortical hyperplasia who had presented with Cushing syndrome at age 2 years (614190), Horvath et al. (2008) identified a heterozygous 914A-C transversion in the PDE8B gene, resulting in a his305-to-pro (H305P) substitution. An adrenal computed tomographic scan showed enlargement of both left and right adrenal glands. Pigment granules were found in some cells of a biopsy specimen of the adrenocortical nodules. The patient inherited the mutation from her father, who was not known to have Cushing syndrome but was obese, had hypertension, and abnormal midnight cortisol levels. Furthermore, the father had mild hyperplasia, especially the left adrenal gland, shown on computed tomography. The mutation was not found in 1,030 unrelated control individuals. The H305P mutation affects an evolutionarily conserved residue. In vitro studies showed significantly higher cAMP levels after transfection with the mutant PDE8B than after transfection with the wildtype PDE8B, indicating an impaired ability of the mutant protein to degrade cAMP.

.0002 STRIATAL DEGENERATION, AUTOSOMAL DOMINANT

In affected members of a large German family with autosomal dominant striatal degeneration (ADSD; 609161), Appenzeller et al. (2010) identified a heterozygous complex mutation in exon 1 of the PDE8B gene: a 94G-C transversion and a 1-bp deletion (95delT) in residue 31, resulting in a premature stop codon after 63 amino acids. The mutation causes loss of all 3 functional domains of PDE8B isoforms 1 through 5, and in vitro functional expression studies in COS-7 cells showed that the mutation resulted in complete loss of enzymatic activity. Further studies in HEK293 and 3T3 cells showed no difference in subcellular localization between the wildtype and mutant protein. The mutant allele was not found in 500 controls. Appenzeller et al. (2010) speculated that the disorder could result from defective dopamine signaling.

NCBI Phenotypes

  • Gene Reviews
  • Striatal degeneration, autosomal dominant
  • Discovery and replication of dopamine-related gene effects on caudate volume in young and elderly populations (N=1198) using genome-wide search.
  • Meta-analysis of two genome-wide association studies identifies four genetic loci associated with thyroid function.
  • GTR
  • OMIM
  • Novel associations for hypothyroidism include known autoimmune risk loci.
  • NHGRI GWA Catalog
  • Pigmented nodular adrenocortical disease, primary, 3

Gene Ontology

  • regulation of transcription, DNA-dependent
  • cyclic nucleotide metabolic process
  • cytosol
  • cellular_component
  • negative regulation of insulin secretion
  • 3’,5’-cyclic-nucleotide phosphodiesterase activity
  • metal ion binding
  • two-component response regulator activity
  • cAMP catabolic process

GeneRIFs

  • Genetic variation in thyroid stimulating hormone levels in pregnancy associated with the PDE8B rs4704397 genotype has implications for the number of women treated for subclinical hypothyroidism under current guidelines [PMID 19820008]
  • Observational study of gene-disease association. (HuGE Navigator) [PMID 19820008]
  • phosphodiesterase 8B has a role in autosomal-dominant striatal degeneration [PMID 20085714]
  • Comparison of enzymatic characterization and gene organization of PDE8B and PDE8A. [PMID 12681444]
  • selective usage of exons produces three different PDE8B variants that exhibit a tissue-specific expression pattern [PMID 12372422]
  • results suggest a primary effect of PDE8B variants on cAMP levels in the thyroid. This would affect production of T4 and T3 and feedback to alter TSH release by the pituitary. [PMID 18514160]
  • PDE8B is another PDE gene linked to isolated micronodular adrenocortical disease; it is a candidate causative gene for other adrenocortical lesions linked to the cAMP signaling pathway and possibly for tumors in other tissues. [PMID 18431404]
  • Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) [PMID 20379614]
  • Our analysis revealed separate segregation of an inactivating PDE8B allele from the high-TSH-allele and showed low TSH levels in persons who carry an inactivating PDE8B allele. These data suggest that PDE8B may be involved in regulation of TSH levels. [PMID 20373981]
  • In obese children, PDE8B is associated with TSH; the interaction between adiposity and PDE8B on TSH is not synergistic, but follows an additive model. [PMID 22084153]
  • Common genetic variation in PDE8B is associated with reciprocal changes in thyroid hormone levels. [PMID 21317282]
  • In Alzheimer’s disease brains we found that PDE8B was the only PDE isozyme showing a significant increase, in cortical areas and parts of the hippocampal formation, at Braak stages III-VI [PMID 12895443]

PubMed Articles

Recent articles:

  • Rawal R et al. “Meta-analysis of two genome-wide association studies identifies four genetic loci associated with thyroid function.” Hum Mol Genet. 2012 Jul 15;21(14):3275-82. PMID 22494929
  • Eriksson N et al. “Novel associations for hypothyroidism include known autoimmune risk loci.” PLoS One. 2012;7(4):e34442. PMID 22493691
  • Grandone A et al. “Impact of phosphodiesterase 8B gene rs4704397 variation on thyroid homeostasis in childhood obesity.” Eur J Endocrinol. 2012 Feb;166(2):255-60. PMID 22084153
  • Stein JL et al. “Discovery and replication of dopamine-related gene effects on caudate volume in young and elderly populations (N=1198) using genome-wide search.” Mol Psychiatry. 2011 Sep;16(9):927-37, 881. PMID 21502949
  • Taylor PN et al. “A meta-analysis of the associations between common variation in the PDE8B gene and thyroid hormone parameters, including assessment of longitudinal stability of associations over time and effect of thyroid hormone replacement.” Eur J Endocrinol. 2011 May;164(5):773-80. PMID 21317282
  • Horvath A et al. “Haplotype analysis of the promoter region of phosphodiesterase type 8B (PDE8B) in correlation with inactivating PDE8B mutation and the serum thyroid-stimulating hormone levels.” Thyroid. 2010 Apr;20(4):363-7. PMID 20373981
  • Rose JE et al. “Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.” Mol Med. 2010 Jul-Aug;16(7-8):247-53. PMID 20379614
  • Appenzeller S et al. “Autosomal-dominant striatal degeneration is caused by a mutation in the phosphodiesterase 8B gene.” Am J Hum Genet. 2010 Jan;86(1):83-7. PMID 20085714
  • Shields BM et al. “Phosphodiesterase 8B gene polymorphism is associated with subclinical hypothyroidism in pregnancy.” J Clin Endocrinol Metab. 2009 Nov;94(11):4608-12. PMID 19820008
  • Bimpaki EI et al. “Abnormalities of cAMP signaling are present in adrenocortical lesions associated with ACTH-independent Cushing syndrome despite the absence of mutations in known genes.” Eur J Endocrinol. 2009 Jul;161(1):153-61. PMID 19429701

Top Pubmed articles linked to gene PDE8B matching any search term:

  • Deninno MP et al. “Discovery of triazolopyrimidine-based PDE8B inhibitors: Exceptionally ligand-efficient and lipophilic ligand-efficient compounds for the treatment of diabetes.” Bioorg Med Chem Lett. 2012 Jul 6;. PMID 22858141
  • Dayan CM et al. “Novel insights into thyroid hormones from the study of common genetic variation.” Nat Rev Endocrinol. 2009 Apr;5(4):211-8. PMID 19352319
  • Waddleton D et al. “Phosphodiesterase 3 and 4 comprise the major cAMP metabolizing enzymes responsible for insulin secretion in INS-1 (832/13) cells and rat islets.” Biochem Pharmacol. 2008 Oct 1;76(7):884-93. PMID 18706893
  • Dov A et al. “Diminished phosphodiesterase-8B potentiates biphasic insulin response to glucose.” Endocrinology. 2008 Feb;149(2):741-8. PMID 17991719

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