pep of 10 Bacillus were downloaded from Ensembl
cat *.* >>99.00spe10_aa.txt

formatdb -i Bacillus_subtilis_subsp_subtilis_str_168.GCA_000009045.1.17.pep.all.fa -p T
blastall -p blastp -d Bacillus_subtilis_subsp_subtilis_str_168.GCA_000009045.1.17.pep.all.fa -i 99.00spe10_aa.txt -o 99.01168_spe10.txt -m 8 -e 10

blastall -p blastp -d Bacillus_subtilis_subsp_subtilis_str_168.GCA_000009045.1.17.pep.all.fa -i Bacillus_alcalophilus_atcc_27647.GCA_000292245.1.19.pep.all.fa -o 99.01168_alc.txt -m 8 -e 10
blastall -p blastp -d Bacillus_subtilis_subsp_subtilis_str_168.GCA_000009045.1.17.pep.all.fa -i Bacillus_azotoformans_lmg_9581.GCA_000307855.1.19.pep.all.fa -o 99.01168_azo.txt -m 8 -e 10
blastall -p blastp -d Bacillus_subtilis_subsp_subtilis_str_168.GCA_000009045.1.17.pep.all.fa -i Bacillus_cellulosilyticus_dsm_2522.GCA_000177235.2.19.pep.all.fa -o 99.01168_cel.txt -m 8 -e 10
blastall -p blastp -d Bacillus_subtilis_subsp_subtilis_str_168.GCA_000009045.1.17.pep.all.fa -i Bacillus_cereus_03bb102.GCA_000022505.1.19.pep.all.fa -o 99.01168_cer.txt -m 8 -e 10
blastall -p blastp -d Bacillus_subtilis_subsp_subtilis_str_168.GCA_000009045.1.17.pep.all.fa -i Bacillus_clausii_ksm_k16.GCA_000009825.1.19.pep.all.fa -o 99.01168_cla.txt -m 8 -e 10
blastall -p blastp -d Bacillus_subtilis_subsp_subtilis_str_168.GCA_000009045.1.17.pep.all.fa -i Bacillus_coagulans_2_6.GCA_000217835.1.19.pep.all.fa -o 99.01168_coa.txt -m 8 -e 10
blastall -p blastp -d Bacillus_subtilis_subsp_subtilis_str_168.GCA_000009045.1.17.pep.all.fa -i Bacillus_halodurans_c_125.GCA_000011145.1.19.pep.all.fa -o 99.01168_hal.txt -m 8 -e 10
blastall -p blastp -d Bacillus_subtilis_subsp_subtilis_str_168.GCA_000009045.1.17.pep.all.fa -i Bacillus_megaterium_dsm_319.GCA_000025805.1.19.pep.all.fa -o 99.01168_meg.txt -m 8 -e 10
blastall -p blastp -d Bacillus_subtilis_subsp_subtilis_str_168.GCA_000009045.1.17.pep.all.fa -i Bacillus_pumilus_atcc_7061.GCA_000172815.1.19.pep.all.fa -o 99.01168_pum.txt -m 8 -e 10
blastall -p blastp -d Bacillus_subtilis_subsp_subtilis_str_168.GCA_000009045.1.17.pep.all.fa -i Bacillus_selenitireducens_mls10.GCA_000093085.1.19.pep.all.fa -o 99.01168_sel.txt -m 8 -e 10

perl 02.best_hit.pl
perl 03.alain.pl
