1128 Cranberry Fraction Induced Alteration of Periodontal Pathogen Lipopolyscacharide Functions

Friday, March 23, 2012: 3:30 p.m. - 4:45 p.m.
Presentation Type: Poster Session
V.S. SANGHA, D. TIPTON, and J. BABU, Periodontics, University of Tennessee, Memphis, TN
Non-dialyzable component isolated from cranberry (Vaccinium macrocarpon) has been studied by various investigators and reported to possess antimicrobial activity, and capable of altering certain outer membrane proteins of periodontal pathogens. However, its effect on biological function of Lipopolysaccharide (LPS) has not been studied well. Objective: The purpose of this investigation was to study the effect of cranberry non dialyzable material (NDM) which is rich in proanthocyanidins, on LPS isolated from periodontal pathogens, P. gingivalis (Pg), P. denticola (Pd) and F. nucleatum (Fn) on cultured THP-1 cells to secrete inflammatory cytokines. Methods: NDM was isolated from cranberry juice concentrate. Monocytes (1x105 cells) were incubated for 48 hours with (10μg/ml) of LPS isolated from Pg, Pd, and Fn grown in the presence or absence of NDM. Cell supernatants were assayed for cytokines, IL-1β and TNF-α using commercial enzyme-linked immunosorbent assay kits. Each experiment was repeated three times with triplicate determinants at each data point. The data were analyzed by one-way analysis of variance, with a level of significance of p <0.05. Results: Isolated LPS (10ng/ml) from three oral pathogens stimulated the secretion of inflammatory cytokines. Pg LPS treated cells secreted 980±184 pg/ml of IL-1β and 1238±188 pg/ml of TNF-α. LPS obtained from cells grown in the presence of NDM stimulated cells to secrete 298±59, and 328±43 pg/ml of IL-1 β and TNF-α, respectively. The LPS of Pd and Fn grown in the presence of NDM secreted about 28% less IL-1b and 34% less of TNF-a, respectively. The results suggest that by suppressing the LPS ability to secrete inflammatory cytokines NDM may be exerting its anti-inflammatory effect. Conclusion: The results demonstrated that cranberry NDM has the potential to interfere with LPS function and may benefit the oral health by suppressing the secretion of inflammatory cytokines.

 Supported by UT College of Dentistry Alumni Foundation

Keywords: Bacterial, Inflammatory mediators, Pedodontics, Periodontal disease and Periodontal organisms