P.F.S.R.L. - AP
Introduction
AP is an extremely stable enzyme, resistant to heat denaturation and to
denaturation by sodium dodecyl sulfate (SDS). It is a
dimeric metalloenzyme, containing 4 Zn atoms and 2 Mg atoms per dimer. In
addition it has recently been shown to possess an intense, long-lived
tryptophan phosphorescence. Although AP has three tryptophans per
monomer, it has been determined that the phosphorescence arises from
Tryptophan 109 (W109), located near the core of the protein. It is this
phosphorescence and the dependence it shows upon the environment of W109
that is our primary tool for studying the folding and stability of AP.
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