Gafni/Steel Selected
Publications
2011
Johnson, R.D., Schauerte, J.A., Wisser, K.C., Gafni, A., Steel, D.G.
Direct Observation of Single Amyloid-beta (1-40) Oligomers on Live Cells: Binding and Growth at Physiological Concentrations.
PLoS One. 2011; 6(8): e23970. (Link)
Brender, J.R., Lee, E.L., Hartman, K., Wong, P.T., Ramamoorthy, A., Steel, D.G., Gafni, A.
Biphasic Effects of Insulin on Islet Amyloid Polypeptide Membrane Disruption.
Biophys J. 2011; 100(3): 685-92. (Link)
2010
Schauerte, J.A., Wong, P.T., Wisser, K.C., Ding, H., Steel, D.G., Gafni, A.
Simultaneous Single-Molecule Fluorescence and Conductivity Studies Reveal Distinct Classes of Abeta Species on Lipid Bilayers.
Biochemistry. 2010; 49(14):3031-9. (Link)
2009
Ding, H., Wong, P.T., Lee, E.L., Gafni, A., Steel, D.G.
Determination of the Oligomer Size of Amyloidogenic Protein Beta-Amyloid(1-40) by Single-Molecule Spectroscopy.
Biophys J. 2009;97(3):912-21. (Link)
Tapley, T.L., Korner, J.L., Barge, M.T., Hupfeld, J., Schauerte, J.A., Gafni, A., Jakob, U., Bardwell, J.C.
Structural Plasticity of an Acid-activated Chaperone Allows Promiscuous Substrate Binding.
Proc Natl Acad Sci U S A. 2009;106(14):5557-62. (Link)
Wong, P.T., Schauerte, J.A., Wisser, K.C., Ding, H., Lee, E.L., Steel, D.G., Gafni, A.
Amyloid-Beta Membrane Binding and Permeabilization are Distinct Processes Influenced Separately by Membrane Charge and Fluidity.
J Mol Biol. 2009;386(1):81-96. (Link)
2008
Shi, J., Dertouzos, J., Gafni, A., Steel, D.
Application of Single-Molecule Spectroscopy in Studying Enzyme Kinetics and Mechanism.
Methods Enzymol. 2008;450:129-57. (Link)
Brender, J.R., Lee, E.L., Cavitt, M.A., Gafni, A., Steel, D.G., Ramamoorthy, A.
Amyloid Fiber Formation and Membrane Disruption are Separate Processes Localized in Two Distinct Regions of IAPP, the Type-2-Diabetes-Related Peptide.
J Am Chem Soc. 2008;130(20):6424-9. (Link)
2006
Shi, J., Gafni, A., Steel, D.
Simulated Data Sets for Single Molecule Kinetics: Some Limitations and Complications of Data Analysis.
Eur Biophys J. 2006;35(8):633-45. (Link)
Shi, J., Dertouzos, J., Gafni, A., Steel, D., Palfey, B.A.
Single-Molecule Kinetics Reveals Signatures of Half-Sites Reactivity in Dihydroorotate Dehydrogenase a Catalysis.
Proc Natl Acad Sci U S A. 2006;103(15):5775-80. (Link)
2005
Brender, J.R., Dertouzos, J., Ballou, D.P., Massey, V., Palfey, B.A., Entsch, B., Steel, D.G., Gafni, A.
Conformational Dynamics of the Isoalloxazine in Substrate-Free P-Hydroxybenzoate Hydroxylase: Single-Molecule Studies.
J Am Chem Soc. 2005;127(51):18171-8. (Link)
Sherman, W.A., Blouse, G.E., Perron, M.J., Tran, T., Shore, J.D., Gafni, A.
Enthalpy Measurement Using Calorimetry Shows a Significant Difference in Potential Energy Between the Active and Latent Conformations of PAI-1.
Biol Chem. 2005;386(2):111-6. (Link)
Before 2005
Shi, J., Palfey,
B.
A., Dertouzos, J., Jensen,
K. F., Gafni, A., Steel,
D. (2004) Multiple States of the Tyr318 Leu Mutant of Dihydroorotate
Dehydrogenase Revealed by Single-Molecule Kinetics J. Am. Chem. Soc. 126(22);
6914-6922.
Wisser, K.C., Schauerte, J.A., Burke,
D.T.,
Galecki, A., Chen, S., Miller, R.A. and Gafni, A. (2004) Mapping
Tissue-Specific Genes Correlated with Age-Dependent Changes in Protein
Stability and Function. Archives
of Biochemistry and Biophysics 432,58-70.
Rhodes, E., and Gafni, A. (2003) Micelle
formation by a fragment
of human islet amyloid polypeptide. Biophys.
J. 84(5):3480-7.
Fisher, C.J., Gafni, A., Steel, D.G., and
Schauerte, J.A. (2002)
The triplet-state lifetime of indole in aqueous and viscous
environments: significance to the interpretation of room
temperature phosphorescence in proteins. J. Am. Chem Soc.
124(35):10359-66.
Dirnbach, E., Steel, D.G., and Gafni, A.
(2001)
Mg2+ binding to
alkaline phosphatase correlates with slow chanes in protein lability. Biochemistry 40(37):11219-26.
Fischer, C.J., Schauerte, J.A., Wisser,
K.C.,
Steel, D.G., and
Gafni, A. (2001) Differences in the pathways for unfolding and hydrogen
exchange among mutants of Escherichia coli alkaline phosphatase. Biochem. Biophys. Acta.
1545(1-2):96-103.
Fischer, C.J., Schauerte, J.A., Wisser,
K.C.,
Gafni, A., and
Steel, D.BG. (2000) Hydrogen exchange at the ore of Escherichia coli
alkaline phosphatase studied by room-temperature phosphorescence. Biochemistry 39(6):1455-61.
Dirnbach, E., Steel, D.G., and Gafni, A.
(1999)
Proline
isomerization is unlikely to be the cause of slow annealing and
reactivation during the folding of alkaline phosphatase. J. Biol. Chem. 274(8):4532-6.
Lewis, M.K., Wolanin, P., Gafni, A.,
Steel, D.G.
(1998)
Near-Field
Scanning Optical Microscopy of Single Molecules by Femtosecond
Two-Photon
Excitation. Optics Letters
23;1111-1113.
Schauerte, J.A., Steel, D.G., and Gafni,
A.
(1997) Time-resolved
room temperature tryptophan phosphorescence in proteins. Methods Enzymol. 278:49-71.
Hansen, J.E., Steel, D.G., and Gafni, A.
(1996)
Detection of a
pH-dependent conformational change in azuring by time-resolved
phosphorescence. Biophys. J. 71(4):2138-43.
Subramaniam, V.,
Steel, D.G., and Gafni, A. (1996) In vitro
renaturation of bovine beta-lactoglobulin A leads to a biologically
active but incompletely refolded state. Protein Sci. 5(10):2089-94.
Schauerte, J.A., Gafni, A., and Steel,
D.G.
(1996) Improved
Differentiation Between Luminescence Decay Components by Use of
Time-Resolved Optical Activity Measurements and Selective Lifetime
Modulation. Biophys. J. 70:1996-2000.
Schauerte, J.A., and Gafni, A. (1995)
Photodegradation of
Tryptophan
Residues and Attenuation of Molecular Chaperone Activity in
Alpha-Crystallin
are Correlated. Biochem. Biophys. Res. Comm. 212:900-905.
Schauerte, J.A.,
Schlyer, B.D., Steel. D.G., Gafni, A. (1995)
Nanosecond Time-Resolved Circular Polarization of Fluorescence: Study
of
NADH Bound to Horse Liver Alcohol Dehydrogenase. Proc. Natl. Acad.
Sci.
USA92:569-573.
Schlyer, B.D., Schauerte, J.A., Steel,
D.G., and
Gafni, A. (1994)
Time-resolved room temperature protein phosphorescence: nonexponential
decay from single emitting tryptophans. Biophys. J.
67:1192-1202.
Hansen, J. and Gafni, A. (1994)
Fluorescence
Detection of
Conformational Changes in GroEL Induced by Thermal Switching and
Nucleotide Binding. J. Biol.
Chem 269:6286-6289.
Mersol, J.V.,Steel, D.G., Gafni, A. (1993)
Detection of
Intermediate
Protein Conformations by Room Temperature Phosphorescence Spectroscopy
During Denaturation of Escherichia Coli Alkaline Phosphatase. Biophysical
Chemistry 48:281-291.
Mersol,
J.V.,Steel, D.G., Gafni, A. (1991) Quenching of
Tryptophan
Phosphorescence in Escherichia Coli Alkaline Phosphatase by Long-Range
Transfer Mechanisms to External Agents in the Rapid Diffusion Limit. Biochemistry
30:668-675.