Experiment Goal
Generating and Using a Calibration Graph
Calibration Graphs are used to determine many things. In this experiment you are using a calibration graph to relate absorbance and concentration. They are used in many fields to relate a quantity with something you can physically measure. In the lab, you will prepare standards of a known concnetration, and plot a calibration graph. You will then use that graph to figure out the concentration of an unknown sample.
Terms you will need to know for the experiment
Dillution
Linear
Straight Line
Line of Best Fit
Absorbance
Concentration
Unknown
Concepts you will learn
What is a dillution factor?
What is a line of best fit?
What is a calibration graph and how do you use it?
Skills you will learn
How to dillute samples
How to generate and plot a calibration graph
How to work with the calibration graph to be able to determine an unknown