Experiment Goal

Generating and Using a Calibration Graph

 

Calibration Graphs are used to determine many things. In this experiment you are using a calibration graph to relate absorbance and concentration. They are used in many fields to relate a quantity with something you can physically measure. In the lab, you will prepare standards of a known concnetration, and plot a calibration graph. You will then use that graph to figure out the concentration of an unknown sample.

 

Terms you will need to know for the experiment

Dillution

Linear

Straight Line

Line of Best Fit

Absorbance

Concentration

Unknown

 

Concepts you will learn

What is a dillution factor?

What is a line of best fit?

What is a calibration graph and how do you use it?

 

Skills you will learn

How to dillute samples

How to generate and plot a calibration graph

How to work with the calibration graph to be able to determine an unknown